Application
Antibody used in WB on Human HepG2 at 1 ug/ml.Application
Antibody used in IHC on Human cornea at 1:100.Application
Antibody used in WB on Human cell lines at: 1:2000 (Lane 1: 25ug MIA PaCa-2 cell lysate, Lane 2: 25ug MDA-MB-231 cell lysate, Lane 3: 25ug Huh-7 cell lysate).| Product Name | NPM1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NPM1, B23, NPM |
| Product description | NPM3 is a member of Nucleophosmin (NPM) family. NPM is a ubiquitously expressed nucleolar phosphoprotein that continuously shuttles between the nucleus and cytoplasm1) Falini, B., et al., (2005) N. Engl. J. Med. 352 (3), 254-266. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NPM1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NPM1 antibody can be used for detection of NPM1 by ELISA at 1:12500. NPM1 antibody can be used for detection of NPM1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHuman |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | NPM1 |
|---|---|
| Alternative Names | NPM1 B23 NPM |
| Molecular Weight(MW) | 29 kDa |
Application
Antibody used in WB on Human HepG2 at 1 ug/ml.
Application
Antibody used in IHC on Human cornea at 1:100.
Application
Antibody used in WB on Human cell lines at: 1:2000 (Lane 1: 25ug MIA PaCa-2 cell lysate, Lane 2: 25ug MDA-MB-231 cell lysate, Lane 3: 25ug Huh-7 cell lysate).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | NPM1 antibody can be used for detection of NPM1 by ELISA at 1:12500. NPM1 antibody can be used for detection of NPM1 by western blot at 1.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NPM1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
