Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.Application
Antibody used in IHC on Human kidney.| Product Name | NR2F2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NR2F2, ARP1, COUP-TFII, COUPTFB, MGC117452, SVP40, TFCOUP2, NF-E3, NR2F1, COUPTFII |
| Product description | NR2F2, an orphan member of the nuclear hormone receptor superfamily, acts as a transcriptional repressor by antagonizing the functions of other nuclear hormone receptors and by actively silencing transcription. However, in certain contexts, NR2F2 stimulates transcription directly. NR2F2, MyoD and p300 interact in a competitive manner, and that increasing amounts of NR2F2 have the ability to reduce the interaction between myoD and p300 invitro. NR2F2 post-transcriptionally regulates myoD activity/function, and that crosstalk between orphan nuclear receptors and the myogenic bHLH proteins has functional consequences for differentiation.1) Schafer, G., (2008) Cancer Res. 68 (2), 457-466. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NR2F2. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NR2F2 antibody can be used for detection of NR2F2 by ELISA at 1:62500. NR2F2 antibody can be used for detection of NR2F2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NR2F2 |
|---|---|
| Alternative Names | NR2F2 ARP1 COUP-TFII COUPTFB MGC117452 SVP40 TFCOUP2 NF-E3 NR2F1 COUPTFII |
| Molecular Weight(MW) | 45 kDa |
Application
Antibody used in WB on Human 293T at 0.2-1 ug/ml.
Application
Antibody used in IHC on Human kidney.| Positive Control | 1) Cat. No. 1219 - MCF7 Cell Lysate |
|---|---|
| Application Notes | NR2F2 antibody can be used for detection of NR2F2 by ELISA at 1:62500. NR2F2 antibody can be used for detection of NR2F2 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NR2F2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
