NIX1 holds two copies of the LXXLL motif. NIX1 displays a neuronal specific expression pattern. It selectively interacts with distinct nuclear receptors of the RAR and TR subfamily, but does not bind steroid hormone receptors. By docking to activated nuclear receptors in an AF2-D-dependent fashion, NIX1 might displace coactivators and result in suppression of the transcriptional activity of liganded nuclear receptors.1) Greiner, E.F., et al., (2000) Proc. Natl. Acad. Sci. U.S.A. 97 (13), 7160-7165.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of mouse NRIP2.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NRIP2 antibody can be used for detection of NRIP2 by ELISA at 1:312500. NRIP2 antibody can be used for detection of NRIP2 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Cerebellum at 0.125 ug/ml.
Positive Control
1) Cat. No. 1362 - Human Cerebellum (Left) Tissue Lysate
Application Notes
NRIP2 antibody can be used for detection of NRIP2 by ELISA at 1:312500. NRIP2 antibody can be used for detection of NRIP2 by western blot at 0.125 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NRIP2 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.