Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of NRP1 Antibody in MDA-MB435 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with NRP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | NRP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Neuropilin-1, Vascular endothelial cell growth factor 165 receptor, CD304, NRP1, NRP, VEGF165R |
| Product description | NRP1 is a membrane-bound coreceptor to a tyrosine kinase receptor for both vascular endothelial growth factor (VEGF; MIM 192240) and semaphorin (see SEMA3A; MIM 603961) family members. NRP1 plays versatile roles in angiogenesis, axon guidance, cell survival, migration, and invasion.1) Hong,J.M., et.al., Exp. Mol. Med. (2010) In press Joslyn,G., et.al., Alcohol. Clin. Exp. Res. (2010) In press |
| Immunogen | This NRP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 722-750 amino acids from the C-terminal region of human NRP1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NRP1 |
|---|---|
| Alternative Names | Neuropilin-1 Vascular endothelial cell growth factor 165 receptor CD304 NRP1 NRP VEGF165R |
| Molecular Weight(MW) | 103 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of MDA-MB435 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of NRP1 Antibody in MDA-MB435 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human skeletal muscle reacted with NRP1 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
