Application
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: RPMI-8226 whole cell lysate Lane 3: SH-SY5Y lysate Lysates/proteins at 20 ug per lane.| Product Name | NT5M Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | 5'(3')-deoxyribonucleotidase, mitochondrial, 5',3'-nucleotidase, mitochondrial, 3.1.3.-, Deoxy-5'-nucleotidase 2, dNT-2, NT5M, DNT2 |
| Product description | Dephosphorylates specifically the 5' and 2'(3')- phosphates of uracil and thymine deoxyribonucleotides, and so protects mitochondrial DNA replication from excess dTTP. Has only marginal activity towards dIMP and dGMP.1) Rampazzo C.,et al.Proc. Natl. Acad. Sci. U.S.A. 97:8239-8244(2000). |
| Immunogen | This NT5M antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 195-226 amino acids from human NT5M. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NT5M |
|---|---|
| Alternative Names | 5'(3')-deoxyribonucleotidase mitochondrial 5' 3'-nucleotidase mitochondrial 3.1.3.- Deoxy-5'-nucleotidase 2 dNT-2 NT5M DNT2 |
| Molecular Weight(MW) | 26 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Western Blot at 1:2000 dilution Lane 1: human liver lysate Lane 2: RPMI-8226 whole cell lysate Lane 3: SH-SY5Y lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
