Application
Western blot analysis of GNL3 Antibody in HL60 cell line lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Product Name | Nucleostemin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Guanine nucleotide-binding protein-like 3, E2-induced gene 3 protein, Novel nucleolar protein 47, NNP47, Nucleolar GTP-binding protein 3, Nucleostemin, GNL3, E2IG3, NS |
| Product description | GNL3 is found in the nucleoli of embryonic stem cells, adult CNS stem cells, primitive cells in the bone marrow and cancer cells. It is not in the differentiated cells of most adult tissues. It has been suggested to play a role in controlling the cell-cycle progression in stem cells and cancer cells, and may be required to maintain the proliferative capacity of stem cells.1) Ma,H.,Mol. Biol. Cell 18 (7), 2630-2635 (2007) |
| Immunogen | This Nucleostemin (GNL3) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 458-487 amino acids from the C-terminal region of human Nucleostemin (GNL3). |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | GNL3 |
|---|---|
| Alternative Names | Guanine nucleotide-binding protein-like 3 E2-induced gene 3 protein Novel nucleolar protein 47 NNP47 Nucleolar GTP-binding protein 3 Nucleostemin GNL3 E2IG3 NS |
| Molecular Weight(MW) | 62 kDa |
Application
Western blot analysis of GNL3 Antibody in HL60 cell line lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma tissue reacted with GNL3 antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
