Human ADP-ribose pyrophosphatase NUDT9 belongs to a superfamily of Nudix hydrolases that catabolize potentially toxic compounds in the cell. NUDT9 alpha protein is targeted highly specifically to mitochondria, whereas the predicted protein of the NUDT9 beta transcript, which is missing this sequence, exhibits no clear subcellular localization. Investigation of the physical and enzymatic properties of NUDT9 indicates that it is functional as a monomer, optimally active at near neutral pH, and that it requires divalent metal ions and an intact Nudix motif for enzymatic activity.1) Clark, H.F., et al., (2003) Genome Res. 13 (10), 2265-2270.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NUDT9.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
NUDT9 antibody can be used for detection of NUDT9 by ELISA at 1:62500. NUDT9 antibody can be used for detection of NUDT9 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
NUDT9 antibody can be used for detection of NUDT9 by ELISA at 1:62500. NUDT9 antibody can be used for detection of NUDT9 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NUDT9 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.