Application
Antibody used in IF on Human HeLa at 2 ug/ml.Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Product Name | NUP35 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NUP35, MP44, NP44, MP-44, NUP53 |
| Product description | NUP35 is a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC. This gene encodes a member of the nucleoporin family. The protein is localized to the nuclear rim and is part of the nuclear pore complex (NPC). All molecules entering or leaving the nucleus either diffuse through or are actively transported by the NPC.1) Olsen, J.V., (2006) Cell 127 (3), 635-648. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NUP35. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| NUP35 antibody can be used for detection of NUP35 by ELISA at 1:1562500. NUP35 antibody can be used for detection of NUP35 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUP35 |
|---|---|
| Alternative Names | NUP35 MP44 NP44 MP-44 NUP53 |
| Molecular Weight(MW) | 35 kDa |
Application
Antibody used in IF on Human HeLa at 2 ug/ml.
Application
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.| Positive Control | 1) Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate |
|---|---|
| Application Notes | NUP35 antibody can be used for detection of NUP35 by ELISA at 1:1562500. NUP35 antibody can be used for detection of NUP35 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store NUP35 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
