Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | NUP85 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Nuclear pore complex protein Nup85, 85 kDa nucleoporin, FROUNT, Nucleoporin Nup75, Nucleoporin Nup85, Pericentrin-1, NUP85, NUP75, PCNT1 |
| Product description | Essential component of the nuclear pore complex (NPC) that seems to be required for NPC assembly and maintenance. As part of the NPC Nup107-160 subcomplex plays a role in RNA export and in tethering NUP98/Nup98 and NUP153 to the nucleus. The Nup107-160 complex seems to be required for spindle assembly during mitosis. NUP85 is required for membrane clustering of CCL2- activated CCR2. Seems to be involved in CCR2-mediated chemotaxis of monocytes and may link activated CCR2 to the phosphatidyl- inositol 3-kinase-Rac-lammellipodium protrusion cascade.1) Cronshaw J.M.,et al.J. Cell Biol. 158:915-927(2002). |
| Immunogen | This NUP85 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 163-195 amino acids from human NUP85. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUP85 |
|---|---|
| Alternative Names | Nuclear pore complex protein Nup85 85 kDa nucleoporin FROUNT Nucleoporin Nup75 Nucleoporin Nup85 Pericentrin-1 NUP85 NUP75 PCNT1 |
| Molecular Weight(MW) | 75 kDa |
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysate Lane 2: Jurkat whole cell lysate Lane 3: K562 whole cell lysate Lane 4: MOLT-4 whole cell lysate Lane 5: NCI-H1299 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
