NUSAP1 is a microtubule-associated protein with the capacity to bundle and stabilize microtubules. It may associate with chromosomes and promote the organization of mitotic spindle microtubules around them.1) Raemaekers, T., (2003) J. Cell Biol. 162 (6), 1017-1029.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human NUSAP1.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
NUSAP1 antibody can be used for detection of NUSAP1 by ELISA at 1:312500. NUSAP1 antibody can be used for detection of NUSAP1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Antibody used in WB on Human Jurkat cells at 0.2-1 ug/ml.
Positive Control
1) Cat. No. 1205 - Jurkat Cell Lysate
Application Notes
NUSAP1 antibody can be used for detection of NUSAP1 by ELISA at 1:312500. NUSAP1 antibody can be used for detection of NUSAP1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store NUSAP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.