Application
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Antibody is used in Western blot to detect NYREN18 in mouse liver lysate.Application
Western blot analysis of NUB1 using NYREN18 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUB1 gene (Lane 2).| Product Name | NYREN18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | NYREN18 is a specific down-regulator of the NEDD8 conjugation system. It recruits NEDD8 and its conjugates to the proteasome for degradation. It also interacts with PSMD4/S5a, a member of the regulatory subunit of the 26S proteasome.1) Tanaka, T., et al., J. Biol. Chem. 278(35):32905-32913 (2003). |
| Immunogen | This NYREN18 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 87-117 amino acids from the N-terminal region of human NYREN18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 | |
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NUB1 |
|---|---|
| Alternative Names | NEDD8 ultimate buster 1 Negative regulator of ubiquitin-like proteins 1 Renal carcinoma antigen NY-REN-18 NUB1 NYREN18 |
| Molecular Weight(MW) | 71 kDa |
Application
Western blot analysis in SK-BR-3 cell line lysates (35ug/lane).
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Antibody is used in Western blot to detect NYREN18 in mouse liver lysate.
Application
Western blot analysis of NUB1 using NYREN18 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the NUB1 gene (Lane 2).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
