Application
Immunohistochemistry of OCC-1 in human spleen tissue with OCC-1 antibody at 2.5 μg/mL.Application
Immunofluorescence of OCC-1 in human spleen tissue with OCC-1 antibody at 20 μg/mL.Application
Western blot analysis of OCC-1 in human spleen tissue lysate with OCC-1 antibody at 2 μg/mL.| Product Name | OCC-1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OCC-1 Antibody: AGD3, OCC1, OCC-1, C12orf75, Overexpressed in colon carcinoma 1 protein, AGD3 |
| Product description | OCC-1 Antibody: OCC-1 (Overexpressed in colon carcinoma-1) was initially identified as a novel human cDNA overexpressed in a colon carcinoma cell line. Elevated levels of OCC-1 mRNA were observed in three of eight colon carcinomas, suggesting that OCC-1 may be a hallmark of only a subset of colon carcinomas. It is highly expressed in skeletal muscle, kidney, placenta, and pancreas tissues, with low to no expression in heart, brain, lung and liver tissues. At least two different OCC-1 mRNA species are known to exist.1) Pibouin L, Villaudy J, Ferbus D, et al. Cloning of the mRNA of overexpression in colon-carcinoma-1: a sequence overexpressed in a subset of colon carcinomas. Cancer Genet. Cytogenet.2002; 133:55-60. |
| Immunogen | OCC-1 antibody was raised against a 16 amino acid synthetic peptide near the amino terminus of human OCC-1. The immunogen is located within amino acids 10 - 60 of OCC-1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| OCC-1 antibody can be used for detection of OCC-1 by Western blot at 2 μg/mL. In vitro transcription and translation of the OCC-1 cDNA yields polypeptides less than 9 kDa. Despite this, OCC-1 from cell lines is detected at ~28 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | C12orf75 |
|---|---|
| Alternative Names | OCC-1 Antibody: AGD3 OCC1 OCC-1 C12orf75 Overexpressed in colon carcinoma 1 protein AGD3 |
| Sequence Similarities | 2) Cat. No. 10-901 - Human Spleen Tissue Slide |
Application
Immunohistochemistry of OCC-1 in human spleen tissue with OCC-1 antibody at 2.5 μg/mL.
Application
Immunofluorescence of OCC-1 in human spleen tissue with OCC-1 antibody at 20 μg/mL.
Application
Western blot analysis of OCC-1 in human spleen tissue lysate with OCC-1 antibody at 2 μg/mL.| Positive Control | 1) Cat. No. 1210 - HEK293 Cell Lysate |
|---|---|
| Application Notes | OCC-1 antibody can be used for detection of OCC-1 by Western blot at 2 μg/mL. In vitro transcription and translation of the OCC-1 cDNA yields polypeptides less than 9 kDa. Despite this, OCC-1 from cell lines is detected at ~28 kDa in SDS-PAGE. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OCC-1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | OCC-1 Antibody is supplied in PBS containing 0.02% sodium azide. |
