Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).Application
OGDH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Product Name | OGDH Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | This gene encodes one subunit of the 2-oxoglutarate dehydrogenase complex. This complex catalyzes the overall conversion of 2-oxoglutarate (alpha-ketoglutarate) to succinyl-CoA and CO(2) during the Krebs cycle. The protein is located in the mitochondrial matrix and uses thiamine pyrophosphate as a cofactor. A congenital deficiency in 2-oxoglutarate dehydrogenase activity is believed to lead to hypotonia, metabolic acidosis, and hyperlactatemia. Alternative splicing results in multiple transcript variants encoding distinct isoforms.1) van Bever, Y., et al. Am. J. Med. Genet.143(7):763-767(2007) |
| Immunogen | This OGDH antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 381-410 amino acids from the C-terminal region of human OGDH. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OGDH |
|---|---|
| Alternative Names | 2-oxoglutarate dehydrogenase mitochondrial 2-oxoglutarate dehydrogenase complex component E1 OGDC-E1 Alpha-ketoglutarate dehydrogenase OGDH |
| Molecular Weight(MW) | 116 kDa |
Application
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Application
OGDH Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human heart tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
