Application
Western blot analysis of op18 in EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.Application
Immunofluorescence of op18 in human brain tissue with op18 antibody at 20 μg/mL.Application
Immunohistochemistry of op18 in human brain tissue with op18 antibody at 2.5 μg/mL.| Product Name | op18 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | op18 Antibody: Lag, SMN, OP18, PP17, PP19, PR22, LAP18, C1orf215, Stathmin, Leukemia-associated phosphoprotein p18, Op18 |
| Product description | op18 Antibody: Op18 belongs to the stathmin family of genes and encodes a ubiquitous cytosolic phosphoprotein that may function as an intracellular relay integrating several signaling pathways such as those involved in cell proliferation and differentiation. Op18 has also been shown to be involved in the regulation of the microtubule filament system by destabilizing microtubules, thereby preventing assembly and promoting the disassembly of microtubules. More recently, op18 has been implicated as a potential target of the ASK1-p38 MAP kinase cascade, suggesting that the ASK1-p38 cascade may regulate microtubule dynamics through op18. Op18 is highly expressed in a wide variety of human malignancies, including leukemia, prostate cancer, ovarian carcinoma, and breast carcinoma, suggesting that op18 may be an ideal target for anti-cancer therapeutics.1) Curmi PA, Gavet O, Charbaut E, et al. Stathmin and its phosphoprotein family: general properties, biochemical and functional interaction with tubulin. Cell Structure and Function 1999; 24:345-57. |
| Immunogen | Op18 antibody was raised against a 20 amino acid synthetic peptide from near the carboxy terminus of human op18. The immunogen is located within the last 50 amino acids of op18. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Op18 antibody can be used for detection of op18 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STMN1 |
|---|---|
| Alternative Names | op18 Antibody: Lag SMN OP18 PP17 PP19 PR22 LAP18 C1orf215 Stathmin Leukemia-associated phosphoprotein p18 Op18 |
| Molecular Weight(MW) | Predicted: 16 kDa Observed: 15 kDa |
| Sequence Similarities | 3) Cat. No. 10-301 - Human Brain Tissue Slide |
Application
Western blot analysis of op18 in EL4 cell lysate with op18 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.
Application
Immunofluorescence of op18 in human brain tissue with op18 antibody at 20 μg/mL.
Application
Immunohistochemistry of op18 in human brain tissue with op18 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | Op18 antibody can be used for detection of op18 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | op18 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | op18 Antibody is supplied in PBS containing 0.02% sodium azide. |
