Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using OSR1 (Phospho-Thr185) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HepG2 cells treated with serum using OSR1 (Phospho-Thr185) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | OSR1 (phospho Thr185) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OXSR1, kinase OSR1, Oxidative-stress responsive 1 |
| Product description | Oxidative-stress responsive 1 gene is located in the vicinity of three others genes - GOLGA4, ITGA9 and HYA22 on chromosome 3. These four genes are considered to be candidate tumor suppressors. Oxidative-stress responsive 1 protein has similarity to human Ste20/oxidant stress response kinase-1 and is thought to be involved in the response to oxidative stress1) Tamari M., J. Hum. Genet. 44:116-120(1999). |
| Immunogen | OSR1 (phospho Thr185) antibody was raised against a peptide sequence around phosphorylation site of threonine185 (R-K-T (p)-F-V) derived from Human OSR1. |
| Modification | p-Thr185 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OXSR1 |
|---|---|
| Alternative Names | OXSR1 kinase OSR1 Oxidative-stress responsive 1 |
| Molecular Weight(MW) | 65 kDa |
| Tissue Specificity | The antibody detects endogenous levels of OSR1 only when phosphorylated at threonine 185. |
| Entrez Gene | 9943 |
|---|---|
| Protein Accession | NP_005100.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using OSR1 (Phospho-Thr185) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HepG2 cells treated with serum using OSR1 (Phospho-Thr185) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
