Application
Immunohistochemistry staining of Osteopontin in brain cortex tissue using Osteopontin Antibody.| Product Name | Osteopontin Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | SPP1, BSPI, BNSP, Bone sialoprotein 1, ETA-1, Nephropontin, OPN, Secreted phosphoprotein 1, SPP-1, SPP1/CALPHA1 fusion, Urinary stone protein, Uropontin, Osteopontin, Bone sialoprotein I |
| Product description | Osteopontin (OPN) is an arginine-glycine-aspartic acid (RGD)-containing glycoprotein that interacts with integrins and CD44 as major receptors. OPN is multifunctional, with activities in cell migration, cell survival, inhibition of calcification, regulation of immune cell function, and control of tumor cell phenotype. The gene encoding OPN is called spp1. Targeting this gene has revealed that while OPN is not necessary for normal embryonic development, fertility, and health under pathogen-free conditions, loss of the protein has significant consequences in several models of injury/disease as diverse as renal injury, viral and bacterial infection, bone remodeling, and tumor growth. OPN has recently been shown to be a novel substrate for two MMPs, MMP-3 (stromelysin-1) and MMP-7 (matrilysin). There are three cleavage sites for MMP-3 in human OPN, two of which are also cleaved by MMP-7 (see cleavage diagram). Biological assays demonstrate that the MMP-cleaved OPN has increased activity in promoting both cell adhesion and migration compared with full-length OPN. In addition, inhibitory reagents were used to show that the same receptors that interact with OPN also mediate interaction of MMP-cleaved OPN with tumor cells. It is suggested that active forms of OPN at sites of tissue injury may be regulated by the activity of proteases including MMPs and that the differences in activity of modified OPN may be explained by differences in binding affinity of integrins or distinct downstream signaling events. |
| Immunogen | Osteopontin antibody was raised against amino acids 80 - 96 of Osteopontin (Human) using a peptide conjugated to KLH using maleimide. A residue of cysteine was added to the amino terminal end to facilitate coupling. |
| Modification | Acetylated |
| Clonality | Polyclonal |
|---|---|
| Isotype | Cocktail |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| Osteopontin antibody can be used in ELISA starting at 1 μg/mL, Western Blot starting at 1 μg/mL, and immunohistochemistry starting at 2.5 μg/mL.: |
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| Species Reactivity | DogHumanMousePigRat |
| Concentration | 1mg/ml |
| Purification | Sterile Filtration |
| Gene Symbol | SPP1 |
|---|---|
| Alternative Names | SPP1 BSPI BNSP Bone sialoprotein 1 ETA-1 Nephropontin OPN Secreted phosphoprotein 1 SPP-1 SPP1/CALPHA1 fusion Urinary stone protein Uropontin Osteopontin Bone sialoprotein I |
| Molecular Weight(MW) | 70KD |
Application
Immunohistochemistry staining of Osteopontin in brain cortex tissue using Osteopontin Antibody.| Application Notes | Osteopontin antibody can be used in ELISA starting at 1 μg/mL, Western Blot starting at 1 μg/mL, and immunohistochemistry starting at 2.5 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store Osteopontin antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.1% sodium azide. |
