Application
Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 μg/mL.Application
Immunofluorescence of OTUD4 in Daudi cells with OTUD4 antibody at 20 μg/mL.Application
Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 μg/mL.| Product Name | OTUD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | OTUD4 Antibody: HIN1, DUBA6, HSHIN1, HIN1, KIAA1046, OTU domain-containing protein 4, HIV-1-induced protein HIN-1 |
| Product description | OTUD4 Antibody: OTUD4, also known as HIV-1 induced protein HIN-1, is a member of the OTU (ovarian tumor) domain containing cysteine protease superfamily, in which the OUT domain generally confers deubiquitinase activity. At least three isoforms of OTUD4 are known to exist, and the smallest of these isoforms are only expressed in HIV-1-infected cells (provided by RefSeq).1) Borodovsky A, Ovaa H, Kolli N, et al. Chemistry-based functional genomics reveals novel members of the deubiquitinating enzyme family. Chem. Biol. 2002; 10:1149-59. |
| Immunogen | OTUD4 antibody was raised against an 18 amino acid synthetic peptide near the carboxy terminus of human OTUD4. The immunogen is located within the last 50 amino acids of OTUD4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | OTUD4 |
|---|---|
| Alternative Names | OTUD4 Antibody: HIN1 DUBA6 HSHIN1 HIN1 KIAA1046 OTU domain-containing protein 4 HIV-1-induced protein HIN-1 |
| Molecular Weight(MW) | Predicted: 115, 123 kDa Observed: 125 kDa |
| Tissue Specificity | Multiple isoforms of OTUD4 are known to exist. OTUD4 antibody is predicted to not cross-react with other members of the OTUD family. |
| Entrez Gene | 54726 |
|---|---|
| Protein Accession | NP_001096123 |
Application
Western blot analysis of OTUD4 in Daudi cell lysate with OTUD4 antibody at (A) 0.25 and (B) 0.5 μg/mL.
Application
Immunofluorescence of OTUD4 in Daudi cells with OTUD4 antibody at 20 μg/mL.
Application
Immunocytochemistry of OTUD4 in Daudi cells with OTUD4 antibody at 2.5 μg/mL.| Positive Control | 1) Cat. No. 1224 - Daudi Cell Lysate |
|---|---|
| Application Notes | OTUD4 antibody can be used for detection of OTUD4 by Western blot at 0.25 - 0.5 μg/mL. Antibody can also be used for immunocytochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | OTUD4 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | OTUD4 Antibody is supplied in PBS containing 0.02% sodium azide. |
