Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1 (Phospho-Thr187).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, IFN-α using p27Kip1 (Phospho-Thr187).| Product Name | p27Kip1 (phospho Thr187) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | KIP1, MEN4, CDKN4, MEN1B, P27KIP1, MAPTL, MTBT1, TAU, CDKN1B, CDN1B, Cyclin-dependent kinase inhibitor 1B, Cyclin-dependent kinase inhibitor p27, KIP1 |
| Product description | Important regulator of cell cycle progression. Involved in G1 arrest. Potent inhibitor of cyclin E- and cyclin A-CDK2 complexes. Positive regulator of cyclin D-dependent kinases such as CDK4. Regulated by phosphorylation and degradation events.1) Eguchi H, et al. (2003) Cancer Res; 63 (15): 4739-46Le XF, et al. (2003) J Biol Chem;Connor MK, et al. (2003) ) Mol Biol Cell; 14 (1): 201-13Fujita N, et al. (2002) J Biol Chem; 277 (32): 28706-13Ishida N, et al. (2000) J Biol Chem; 275 (33): 25146-54 |
| Immunogen | p27Kip1 (Phospho-Thr187) antibody was raised against a peptide sequence around phosphorylation site of threonine 187 (E-Q-T (p) -P-K) derived from Human p27Kip1. |
| Modification | p-Thr187 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | CDKN1B |
|---|---|
| Alternative Names | KIP1 MEN4 CDKN4 MEN1B P27KIP1 MAPTL MTBT1 TAU CDKN1B CDN1B Cyclin-dependent kinase inhibitor 1B Cyclin-dependent kinase inhibitor p27 KIP1 |
| Molecular Weight(MW) | 27 kDa |
| Tissue Specificity | This antibody detects endogenous level of p27Kip1 only when phosphorylated at threonine 187. |
| Entrez Gene | 1027 |
|---|---|
| Protein Accession | NP_004055.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p27Kip1 (Phospho-Thr187).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with EGF, IFN-α using p27Kip1 (Phospho-Thr187).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
