Application
Immunocytochemistry of P2RX7 in 3T3 cells with P2RX7 antibody at 5 μg/mL.Application
Immunofluorescence of P2RX7 in 3T3 cells with P2RX7 antibody at 20 μg/mL.Application
Western blot analysis of P2RX7 in 3T3 cell lysate with P2RX7 antibody at (A) 1 and (B) 2 μg/ml.| Product Name | P2RX7 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P2X purinoceptor 7, P2X7, purinergic receptor P2X ligand-gated ion channel 7 |
| Product description | The purinergic receptor P2X ligand-gated ion channel 7 (P2RX7) belongs to the family of purinoceptors for ATP (1). This receptor functions as a ligand-gated ion channel and is responsible for ATP-dependent lysis of macrophages through the formation of membrane pores permeable to large molecules (1,2). Activation of this nuclear receptor by ATP in the cytoplasm may be a mechanism by which cellular activity can be coupled to changes in gene expression (2). Recent studies have suggested that P2RX7 may play a key role in immune-mediated diseases such as rheumatoid arthritis (3) as well as neuropsychiatric disorders (4).1) Surprenant A, Rassendren F, Kawashima E, et al. The cytosolic P2Z receptor for extracellular ATP identified as a P2X receptor (P2X7). Science 1996; 272:735-8. |
| Immunogen | P2RX7 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human P2RX7. The immunogen is located within the last 50 amino acids of P2RX7. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | P2RX7 |
|---|---|
| Alternative Names | P2X purinoceptor 7 P2X7 purinergic receptor P2X ligand-gated ion channel 7 |
| Molecular Weight(MW) | Predicted: 34, 47, 65 kDa Observed: 54 kDa |
| Sequence Similarities | 2) Cat. No. 17-201 - 3T3/BALB Cell Slide |
| Tissue Specificity | P2RX7 antibody is human, mouse, and rat reactive. Multiple isoforms of P2RX7 are known to exist. |
Application
Immunocytochemistry of P2RX7 in 3T3 cells with P2RX7 antibody at 5 μg/mL.
Application
Immunofluorescence of P2RX7 in 3T3 cells with P2RX7 antibody at 20 μg/mL.
Application
Western blot analysis of P2RX7 in 3T3 cell lysate with P2RX7 antibody at (A) 1 and (B) 2 μg/ml.| Positive Control | 1) Cat. No. 1212 - 3T3 Cell Lysate |
|---|---|
| Application Notes | P2RX7 antibody can be used for detection of P2RX7 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | P2RX7 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. |
| Storage Buffer | P2RX7 antibody is supplied in PBS containing 0.02% sodium azide. |
