Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK (Phospho-Thr180).Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with UV using P38 MAPK (Phospho-Thr180).| Product Name | P38 MAPK (phospho Thr180) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, PLC1, MAPK2, MAPKAPK-2, MAPKAPK2 |
| Product description | Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.1) Kim JE, et al. (2005) J Proteome Res Jul-Aug; 4 (4): 1339-1346Meng F, et al. (2005) Am J Physiol Cell Physiol May 25Jin ZH, et al. (2005) Oncogene Mar 17; 24 (12): 1973-1981 |
| Immunogen | P38 MAPK (Phospho-Thr180) antibody was raised against a peptide sequence around phosphorylation site of threonine 180 (E-M-T (p) -G-Y ) derived from Human P38MAPK. |
| Modification | p-Thr180 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK14 |
|---|---|
| Alternative Names | RK p38 CSBP EXIP Mxi2 CSBP1 CSBP2 CSPB1 PRKM14 PRKM15 SAPK2A p38ALPHA PLC1 MAPK2 MAPKAPK-2 MAPKAPK2 |
| Molecular Weight(MW) | 43 kDa |
| Tissue Specificity | This antibody detects endogenous level of P38 MAPK only when phosphorylated at Threonine 180. |
| Entrez Gene | 1432 |
|---|---|
| Protein Accession | NP_001306.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK (Phospho-Thr180).
Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with UV using P38 MAPK (Phospho-Thr180).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
