Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK (Phospho-Tyr182).Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182).| Product Name | P38 MAPK (phospho Tyr182) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RK, p38, CSBP, EXIP, Mxi2, CSBP1, CSBP2, CSPB1, PRKM14, PRKM15, SAPK2A, p38ALPHA, FLK1, VEGFR2, MAPK2, MAPKAPK-2, MAPKAPK2 |
| Product description | Responds to activation by environmental stress, pro-inflammatory cytokines and lipopolysaccharide (LPS) by phosphorylating a number of transcription factors, such as ELK1 and ATF2 and several downstream kinases, such as MAPKAPK2 and MAPKAPK5. Plays a critical role in the production of some cytokines, for example IL-6. May play a role in stabilization of EPO mRNA during hypoxic stress. Isoform Mxi2 activation is stimulated by mitogens and oxidative stress and only poorly phosphorylates ELK1 and ATF2. Isoform Exip may play a role in the early onset of apoptosis.1) Ming Zheng, et al. (2005) The FASEB Journal. 19: 109-111Bernt van den et al. (2001) Blink Immunology, 166: 582-587Arshad Rahman, et al. (2004) Am J Physiol Lung Cell Mol Physiol 287: L1017-L1024Osamu Yoshino, et al. (2003) Endocrinology & Metabolism Vol. 88: 2236-2241 |
| Immunogen | P38 MAPK (Phospho-Tyr182) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 182 (T-G-Y (p) -V-A) derived from Human P38 MAPK. |
| Modification | p-Tyr182 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | MAPK14 |
|---|---|
| Alternative Names | RK p38 CSBP EXIP Mxi2 CSBP1 CSBP2 CSPB1 PRKM14 PRKM15 SAPK2A p38ALPHA FLK1 VEGFR2 MAPK2 MAPKAPK-2 MAPKAPK2 |
| Molecular Weight(MW) | 43 kDa |
| Tissue Specificity | This antibody detects endogenous level of P38MAPK only when phosphorylated at tyrosine 182. |
| Entrez Gene | 1432 |
|---|---|
| Protein Accession | NP_001306.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using P38 MAPK (Phospho-Tyr182).
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with UV for the indicated times, using P38 MAPK (Phospho-Tyr182).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
