Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | p47 phox (phospho Ser345) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NCF1, P47 phox, NCF-47K |
| Product description | NCF2, NCF1, and a membrane bound cytochrome b558 are required for activation of the latent NADPH oxidase (necessary for superoxide production).1) Volpp B.D., Proc. Natl. Acad. Sci. U.S.A. 86:7195-7199(1989). |
| Immunogen | p47 phox (phospho Ser345) antibody was raised against a peptide sequence around phosphorylation site of Serine 345 (P-Q-S (p)-P-G) derived from Human Nuclear p47 phox. |
| Modification | p-Ser345 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NCF1 |
|---|---|
| Alternative Names | NCF1 P47 phox NCF-47K |
| Molecular Weight(MW) | 45 kDa |
| Tissue Specificity | The antibody detects endogenous levels of p47 phox only when phosphorylated at serine 345. |
| Entrez Gene | 653361 |
|---|---|
| Protein Accession | NP_000256.4 |
Application
Immunohistochemical analysis of paraffin-embedded human brain tissue using p47 phox (Phospho-Ser345) antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HepG2 cells treated with TNF using p47 phox (Phospho-Ser345) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
