Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Ab-15). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p53 (Ab-15).Application
Western blot analysis of lysed extracts from MDA and JK cells using p53 (Ab-15).| Product Name | p53 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P53, BCC7, LFS1, TRP53, GRB1, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13, TP53 |
| Product description | p53 is a nuclear protein which plays an essential role in the regulation of cell cycle specifically in the transition from G0 to G1. It is found in very low levels in normal cells however in a variety of transformed cell lines in high amounts and believed to contribute to transformation and malignancy. The open reading frame of p53 is 393 amino acids long, with the central region (consisting of amino acids from about 100 to 300) containing the DNA-binding domain. This proteolysis-resistant core is flanked by a C-terminal end mediating oligomerization and an N-terminal end containing a strong transcription activation signal. p53 binds as a tetramer to a PBS (p53-Binding Site) and activates the expression of downstream genes that inhibit growth and/or invasion. p53 binds as a tetramer to a p53-binding site (PBS) and to activate the expression of adjacent genes that inhibit growth and/or invasion. Deletion of one or both p53 alleles reduces the expression of tetramers, resulting in decreased expression of the growth inhibitory genes1) Lin T, et al. (2005) Nat Cell Biol; 7 (2): 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23): 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40): 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38): 39584-92. |
| Immunogen | p53 (Ab-15) antibody was raised against a peptide sequence around aa.13~17 (P-L-S-Q-E) derived from Human p53. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IFIHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
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| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP53 |
|---|---|
| Alternative Names | P53 BCC7 LFS1 TRP53 GRB1 Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 TP53 |
| Molecular Weight(MW) | 53 kDa |
| Tissue Specificity | This antibody detects endogenous level of total p53 protein. |
| Entrez Gene | 7157 |
|---|---|
| Protein Accession | NP_000537.3 |
Application
Top Image: Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Ab-15). Bottom Image: Immunofluorescence staining of methanol-fixed HeLa cells using p53 (Ab-15).
Application
Western blot analysis of lysed extracts from MDA and JK cells using p53 (Ab-15).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100, Immunofluorescence: 1:100~1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
