Application
Western blot analysis of lysed extracts from HT29 cells using p53 (Ab-18).| Product Name | p53 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P53, BCC7, LFS1, TRP53, MTC, TRK, TRKA, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13 |
| Product description | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.1) Lin T, et al. (2005) Nat Cell Biol; 7 (2): 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23): 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40): 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38): 39584-92. |
| Immunogen | p53 (Ab-18) antibody was raised against a peptide sequence around aa. 16~20 (Q-E-T-F-S) derived from Human p53. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP53 |
|---|---|
| Alternative Names | P53 BCC7 LFS1 TRP53 MTC TRK TRKA Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 |
| Molecular Weight(MW) | 53 kDa |
| Tissue Specificity | This antibody detects endogenous level of total p53 protein. |
| Entrez Gene | 7157 |
|---|---|
| Protein Accession | NP_000537.3 |
Application
Western blot analysis of lysed extracts from HT29 cells using p53 (Ab-18).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
