Application
Western blot analysis of lysed extracts from HT29 cells using p53 (Ab-46).| Product Name | p53 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P53, BCC7, LFS1, TRP53, CD49D, Tumor suppressor p53, Phosphoprotein p53, Antigen NY-CO-13 |
| Product description | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.1) Dhavan, R. and Tsai, L. H. (2001) Nat Rev Mol Cell Biol. 2: 749-759. Patrick, G. N. et al. (1998) J Biol Chem. 273: 24057-24064. Di Stefano V, et al. (2005) Oncogene. 24 (35): 5431-5442. Mayo LD, et al. (2005) J Biol Chem. 280 (28): 25953-25959. Wang L, et al. (2005) Oncogene. 24 (18): 3020-3027. |
| Immunogen | p53 (Ab-46) antibody was raised against a peptide sequence around aa. 44~48 (M-L-S-P-D) derived from Human p53. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP53 |
|---|---|
| Alternative Names | P53 BCC7 LFS1 TRP53 CD49D Tumor suppressor p53 Phosphoprotein p53 Antigen NY-CO-13 |
| Molecular Weight(MW) | 53 kDa |
| Tissue Specificity | This antibody detects endogenous level of total p53 protein. |
| Entrez Gene | 7157 |
|---|---|
| Protein Accession | NP_000537.3 |
Application
Western blot analysis of lysed extracts from HT29 cells using p53 (Ab-46).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
