Application
Western blot analysis of extracts from 293 cells treated with Etoposide using Phospho-p53 (Ser392) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | p53 (phospho Ser392) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Antigen NY-CO-13, Cellular tumor antigen p53, Phosphoprotein p53, TP53, Tumor suppressor p53 |
| Product description | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.1) Choi DW, et al. (2013) Mol Cell 51, 374-85 |
| Immunogen | p53 (phospho Ser392) antibody was raised against a peptide sequence around phosphorylation site of serine 392 (P-D-S (p)-D) derived from Human p53. |
| Modification | p-Ser392 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP53 |
|---|---|
| Alternative Names | Antigen NY-CO-13 Cellular tumor antigen p53 Phosphoprotein p53 TP53 Tumor suppressor p53 |
| Molecular Weight(MW) | 53 kDa |
| Tissue Specificity | The antibody detects endogenous level of p53 only when phosphorylated at serine 392. |
| Entrez Gene | 7157 |
|---|---|
| Protein Accession | NP_000537.3 |
Application
Western blot analysis of extracts from 293 cells treated with Etoposide using Phospho-p53 (Ser392) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
