Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser6).Application
Western blot analysis of lysed extracts from HT29 and HeLa cells untreated or treated with UV using p53 (Phospho-Ser6).| Product Name | p53 (phospho Ser6) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P53, BCC7, LFS1, TRP53, CHK1, Antigen NY-CO-13, Phosphoprotein p53, TP53, Tumor suppressor p53 |
| Product description | Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.1) Lin T, et al. (2005) Nat Cell Biol; 7 (2): 165-71. Vega FM, et al. (2004) Mol Cell Biol; 24 (23): 10366-80. Li J, et al. (2004) J Biol Chem; 279 (40): 41275-9. Wang J, et al. (2004) J Biol Chem; 279 (38): 39584-92. |
| Immunogen | p53 (Phospho-Ser6) antibody was raised against a peptide sequence around phosphorylation site of serine 6 (P-Q-S (p) -D-P) derived from Human p53. |
| Modification | p-Ser6 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP53 |
|---|---|
| Alternative Names | P53 BCC7 LFS1 TRP53 CHK1 Antigen NY-CO-13 Phosphoprotein p53 TP53 Tumor suppressor p53 |
| Molecular Weight(MW) | 53 kDa |
| Tissue Specificity | This antibody detects endogenous level of p53 only when phosphorylated at serine 6. |
| Entrez Gene | 7157 |
|---|---|
| Protein Accession | NP_000537.3 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p53 (Phospho-Ser6).
Application
Western blot analysis of lysed extracts from HT29 and HeLa cells untreated or treated with UV using p53 (Phospho-Ser6).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
