Application
Western blot analysis in 293 cell line lysates (35ug/lane).Application
Immunohistochemical analysis of paraffin-embedded H. esophagus section using p53 Antibody (Sumoylation Site Specific). Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | p53 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53 |
| Product description | Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. P53 is subject to modification by conjugation of SUMO-1. A p53 mutant deficient for MDM2 binding is poorly sumoylated in vivo compared to wild-type p53. Overexpression of MDM2 increases the level of p53 sumoylation, which is further stimulated by expression of ARF. These results show that p53 sumoylation is regulated by MDM2- and ARF-mediated nucleolar targeting.1) Chen L, et al. Oncogene. 2003 Aug 14;22(34):5348-57. |
| Immunogen | This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 364-393 amino acids from human p53. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:100: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | TP53 |
|---|---|
| Alternative Names | Cellular tumor antigen p53 Antigen NY-CO-13 Phosphoprotein p53 Tumor suppressor p53 TP53 P53 |
| Molecular Weight(MW) | 44 kDa |
Application
Western blot analysis in 293 cell line lysates (35ug/lane).
Application
Immunohistochemical analysis of paraffin-embedded H. esophagus section using p53 Antibody (Sumoylation Site Specific). Antibody was diluted at 1:100 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Application
Western Blot at 1:2000 dilution Lane 1: A431 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:2000 For IHC-P starting dilution is: 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
