Application
Western Blot at 1:1000-1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | p53 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Cellular tumor antigen p53, Antigen NY-CO-13, Phosphoprotein p53, Tumor suppressor p53, TP53, P53 |
| Product description | Tumor protein p53, a nuclear protein, plays an essential role in the regulation of cell cycle, specifically in the transition from G0 to G1. It is found in very low levels in normal cells, however, in a variety of transformed cell lines, it is expressed in high amounts, and believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing DNA-binding, oligomerization and transcription activation domains. It is postulated to bind as a tetramer to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of the TP53 gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome.1) Blanchette, P., et al., Mol. Cell. Biol. 24(21):9619-9629 (2004). |
| Immunogen | This p53 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 354-385 amino acids from the C-terminal region of human p53. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000-1:2000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP53 |
|---|---|
| Alternative Names | Cellular tumor antigen p53 Antigen NY-CO-13 Phosphoprotein p53 Tumor suppressor p53 TP53 P53 |
| Molecular Weight(MW) | 44 kDa |
Application
Western Blot at 1:1000-1:2000 dilution Lane 1: 293T/17 whole cell lysate Lane 2: A431 whole cell lysate Lane 3: SK-BR-3 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western Blot at 1:2000 dilution Lane 1: 293 whole cell lysate Lane 2: HT-29 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000-1:2000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
