Application
Western blot analysis of lysed extracts from HT29 and 293 cells using p73 (Ab-99).| Product Name | p73 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | P73, TP73, TRP73, p53-related protein, p73-alpha |
| Product description | This gene encodes tumor protein p73, which is a member of the p53 family of transcription factors involved in cellular responses to stress and development. The family members include p53, p63, and p73 and have high sequence similarity to one another, which allows p63 and p73 to transactivate p53-responsive genes causing cell cycle arrest and apoptosis. The family members can interact with each other in many ways involving direct or indirect protein interactions, resulting in regulation of the same target gene promoters or regulation of each other's promoters. The p73 protein is expressed at very low levels in normal tissues and is differentially expressed in a number of tumors. The p73 gene expresses at least 35 mRNA variants due to the use of alternate promoters, alternate translation initiation sites, and multiple splice variations. Theoretically this can account for 29 different p73 isoforms; however, the biological validity and the full-length nature of most variants have not been determined.1) Yuan ZM, et al. (1999) Nature. 399 (6738): 814-817. |
| Immunogen | p73 (Ab-99) antibody was raised against a peptide sequence around aa.97~101 (S-P-Y-A-Q) derived from Human P73. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | TP73 |
|---|---|
| Alternative Names | P73 TP73 TRP73 p53-related protein p73-alpha |
| Molecular Weight(MW) | 80 kDa |
| Tissue Specificity | This antibody detects endogenous level of total p73 protein. |
| Entrez Gene | 7161 |
|---|---|
| Protein Accession | NP_001119712.1 |
Application
Western blot analysis of lysed extracts from HT29 and 293 cells using p73 (Ab-99).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
