Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with Hydroxyurea using p95/NBS1 (Phospho-Ser343).| Product Name | p95 / NBS1 (phospho Ser343) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | ATV, NBS, P95, NBS1, AT-V1, AT-V2, TCF5, PP9092, |
| Product description | Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation.1) Hsu HL, et al (2005) Oncogene; 24 (31): 4956-64. Falck J, et al. (2005) Nature; 434 (7033): 605-11. Buscemi G, et al. (2004) Oncogene; 23 (46): 7691-700. Beausoleil SA, et al. (2004) Proc Natl Acad Sci U S A; 101 (33): 12130-5. |
| Immunogen | p95/NBS1 (Phospho-Ser343) antibody was raised against a peptide sequence around phosphorylation site of serine 343 (S-L-S (p) -Q-G) derived from Human p95/NBS1. |
| Modification | Phosphoralated |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | NBN |
|---|---|
| Alternative Names | ATV NBS P95 NBS1 AT-V1 AT-V2 TCF5 PP9092 |
| Molecular Weight(MW) | 95 kDa |
| Tissue Specificity | This antibody detects endogenous level of p95/NBS1 only when phosphorylated at serine 343. |
| Entrez Gene | 4683 |
|---|---|
| Protein Accession | NP_002476.2 |
Application
Western blot analysis of lysed extracts from HeLa cells untreated or treated with Hydroxyurea using p95/NBS1 (Phospho-Ser343).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
Data sheet for OM287224