Application
Western blot analysis of PAF1 using rabbit polyclonal PAF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.Application
Western blot analysis in Hela cell line lysates (35ug/lane).| Product Name | PAF1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | RNA polymerase II-associated factor 1 homolog, hPAF1, Pancreatic differentiation protein 2, PAF1, PD2 |
| Product description | PAF1, parafibromin (CDC73; MIM 607393), LEO1 (MIM 610507), and CTR9 (MIM 609366) form the PAF protein complex that associates with the RNA polymerase II subunit POLR2A (MIM 180660) and with a histone methyltransferase complex (Rozenblatt-Rosen et al., 2005 [PubMed 15632063]). The PAF complex also has a role in histone monoubiquitination (Zhu et al., 2005 [PubMed 16307923]).[supplied by OMIM].1) Muntean, A.G., et al. Cancer Cell 17(6):609-621(2010) |
| Immunogen | This PAF1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 439-467 amino acids from the C-terminal region of human PAF1. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB starting dilution is: 1:1000: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAF1 |
|---|---|
| Alternative Names | RNA polymerase II-associated factor 1 homolog hPAF1 Pancreatic differentiation protein 2 PAF1 PD2 |
| Molecular Weight(MW) | 60 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Bovine |
Application
Western blot analysis of PAF1 using rabbit polyclonal PAF1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAF1 gene.
Application
Western blot analysis in Hela cell line lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
