Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK2 (Phospho-Ser197) antibody (left) or the same antibody preincubated with blocking peptide (right).| Product Name | PAK2 (phospho Ser197) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | gamma-PAK, PAK65, PAKI, PAK 2 |
| Product description | The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.1) Martin G.A., EMBO J. 14:1970-1978(1995). |
| Immunogen | PAK2 (phospho Ser197) antibody was raised against a peptide sequence around phosphorylation site of Serine 197 (T-R-S (p)-V-I) derived from Human PAK2. |
| Modification | p-Ser197 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC |
| IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK2 |
|---|---|
| Alternative Names | gamma-PAK PAK65 PAKI PAK 2 |
| Molecular Weight(MW) | 58 kDa |
| Tissue Specificity | The antibody detects endogenous levels of PAK2 only when phosphorylated at serine 197. |
| Entrez Gene | 5062 |
|---|---|
| Protein Accession | NP_002568.2 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue, using PAK2 (Phospho-Ser197) antibody (left) or the same antibody preincubated with blocking peptide (right).| Application Notes | IHC:1:50~1:200 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
