Application
Immunohistochemistry of PAK2 in mouse spleen tissue with PAK2 antibody at 10 μg/mL.Application
Immunofluorescence of PAK2 in mouse spleen tissue with PAK2 antibody at 20 μg/mL.Application
Western blot analysis of PAK2 in Jurkat lysate with PAK2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Product Name | PAK2 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PAK2 Antibody: PAK65, PAKgammaGamma-PAK, PAK-2 |
| Product description | PAK2 Antibody: The p21-activated kinases (PAKs) are serine-threonine kinases that bind to the active forms of Cdc42 and Rac. They are divided into two groups, the first of which include PAK1, 2 and 3, and can be activated by Cdc42/Rac binding. Group 1 PAKs contain an autoinhibitory domain whose activity is regulated by Cdc42/Rac binding. The group 1 PAKs are known to be involved in cellular processes such as gene transcription, apoptosis, and cell morphology and motility. Much less is known about the second group, which includes PAK4, 5 and 6, and are not activated by Cdc42/Rac binding. Of the six PAK proteins, only PAK2 is ubiquitously expressed and cleaved by caspase-3. This cleavage removes the amino-terminal regulatory domain and generates a constitutively active kinase fragment. Recent experiments have shown that following cleavage, the active fragment is myristoylated and directed to the plasma membrane and membrane ruffles where it promotes cell death via increased signaling through the c-Jun N-terminal kinase pathway, but without compromising mitochondrial integrity.1) Jaffer ZM and Chernoff J. p21-activated kinases: three more join the Pak. Int. J. Biochem. Cell Biol. 2002; 34:713-7. |
| Immunogen | PAK2 antibody was raised against a 14 amino acid synthetic peptide from near the amino terminus of human PAK2. The immunogen is located within amino acids 20 - 70 of PAK2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIFIHC-PWB |
| PAK2 antibody can be used for the detection of PAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAK2 |
|---|---|
| Alternative Names | PAK2 Antibody: PAK65 PAKgammaGamma-PAK PAK-2 |
| Sequence Similarities | 2) Cat. No. 1406 - Mouse Spleen Tissue Lysate |
Application
Immunohistochemistry of PAK2 in mouse spleen tissue with PAK2 antibody at 10 μg/mL.
Application
Immunofluorescence of PAK2 in mouse spleen tissue with PAK2 antibody at 20 μg/mL.
Application
Western blot analysis of PAK2 in Jurkat lysate with PAK2 antibody at (A) 0.5, (B) 1 and (C) 2 μg/mL.| Positive Control | 1) Cat. No. 1205 - Jurkat Cell Lysate |
|---|---|
| Application Notes | PAK2 antibody can be used for the detection of PAK2 by Western blot at 0.5 - 2 μg/mL. Antibody can also be used for immunohistochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PAK2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PAK2 Antibody is supplied in PBS containing 0.02% sodium azide. |
