PAOX belongs to the flavin monoamine oxidase family. PAOX is a flavoenzyme which catalyzes the oxidation of N (1)-acetylspermine to spermidine and is thus involved in the polyamine back-conversion. It can also oxidize N (1)-acetylspermidine to putrescine. PAOX does not oxidize spermidine. It plays an important role in the regulation of polyamine intracellular concentration and has the potential to act as a determinant of cellular sensitivity to the antitumor polyamine analogs.1) Jarvinen, A., (2006) J. Biol. Chem. 281 (8), 4589-4595.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAOX.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
PAOX antibody can be used for detection of PAOX by ELISA at 1:312500. PAOX antibody can be used for detection of PAOX by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
PAOX antibody can be used for detection of PAOX by ELISA at 1:312500. PAOX antibody can be used for detection of PAOX by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.:
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAOX antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.