Application
Flow cytometric analysis of widr cells using PAX4 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PAX4 antibody in CEM cell line lysates (35ug/lane)Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: rat liver lysates Lysates/proteins at 20 ug per lane.| Product Name | PAX4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PAX4 is a member of the paired box (PAX) family of transcription factors. These proteins play critical roles during fetal development and cancer growth. The paired box 4 gene is involved in pancreatic islet development and mouse studies have demonstrated a role for this gene in differentiation of insulin-producing beta cells.1) Hata,S., Int. J. Oncol. 33 (5), 1065-1071 (2008) |
| Immunogen | This PAX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 171-200 amino acids from the Central region of human PAX4. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Unpurified |
| Gene Symbol | PAX4 |
|---|---|
| Alternative Names | Paired box protein Pax-4 PAX4 |
| Molecular Weight(MW) | 38 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of widr cells using PAX4 Antibody (bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human brain tissue reacted with PAX4 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PAX4 antibody in CEM cell line lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution Lane 1: Hela whole cell lysates Lane 2: rat liver lysates Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
