The PAX5 gene is a member of the paired box (PAX) family of transcription factors. The central feature of this gene family is a novel, highly conserved DNA-binding motif, known as the paired box. The PAX proteins are important regulators in early development, and alterations in the expression of their genes are thought to contribute to neoplastic transformation. The PAX5 gene encodes the B-cell lineage specific activator protein (BSAP) that is expressed at early, but not late stages of B-cell differentiation. Its expression has also been detected in developing CNS and testis, therefore, PAX5 gene product may not only play an important role in B-cell differentiation, but also in neural development and spermatogenesis.1) Robichaud, G.A., et al., (2004) J. Biol. Chem. 279 (48), 49956-49963.
Immunogen
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PAX5.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAIHCWB
PAX5 antibody can be used for detection of PAX5 by ELISA at 1:312500. PAX5 antibody can be used for detection of PAX5 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
PAX5 antibody can be used for detection of PAX5 by ELISA at 1:312500. PAX5 antibody can be used for detection of PAX5 by western blot at 2.0 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Additional Information
Form
Liquid
Storage Instructions
For short periods of storage (days) store at 4˚C. For longer periods of storage, store PAX5 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Storage Buffer
Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL.