Application
Confocal immunofluorescent analysis of PAX6-T373 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).Application
PAX6-T373 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western blot analysis of PAX6 using rabbit polyclonal PAX6 Antibody (T373) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.Application
Western blot analysis of PAX6-T373 in Y79 cell line lysates (35ug/lane)Application
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.| Product Name | PAX6-T373 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PAX6 encodes paired box gene 6, one of many human homologs of the Drosophila melanogaster gene prd. In addition to the hallmark feature of this gene family, a conserved paired box domain, the encoded protein also contains a homeo box domain. Both domains are known to bind DNA, and function as regulators of gene transcription. This gene is expressed in the developing nervous system, and in developing eyes. Mutations in this gene are known to cause ocular disorders such as aniridia and Peter's anomaly.1) Zhang, Y., et al. J. Biol. Chem. 285(4):2527-2536(2010) |
| Immunogen | This PAX6 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 352-380 amino acids from human PAX6. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IFIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 | |
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAX6 |
|---|---|
| Alternative Names | Paired box protein Pax-6 Aniridia type II protein Oculorhombin PAX6 AN2 |
| Molecular Weight(MW) | 47 kDa |
Application
Confocal immunofluorescent analysis of PAX6-T373 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).
Application
PAX6-T373 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western blot analysis of PAX6 using rabbit polyclonal PAX6 Antibody (T373) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PAX6 gene.
Application
Western blot analysis of PAX6-T373 in Y79 cell line lysates (35ug/lane)
Application
Western Blot at 1:2000 dilution + rat eyeball lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For IF starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
