Application
Flow cytometric analysis of 293 cells using PAX8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.Application
Western blot analysis of PAX8 Antibody in HL-60 cell line lysates (35ug/lane)Application
Western Blot at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.| Product Name | PAX8 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | PAX8 is a member of the paired box (PAX) family of transcription factors. Members of this family contain a paired box domain, an octapeptide, and a paired-type homeodomain. This nuclear protein is involved in thyroid follicular cell development and expression of thyroid-specific genes.1) Ramos,H.E., et.al., Thyroid 19 (1), 61-68 (2009) |
| Immunogen | This PAX8 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 285-313 amino acids from the Central region of human PAX8. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 | |
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PAX8 |
|---|---|
| Alternative Names | Paired box protein Pax-8 PAX8 |
| Molecular Weight(MW) | 48 kDa |
| Sequence Similarities | Predicted species reactivity based on immunogen sequence: Mouse |
Application
Flow cytometric analysis of 293 cells using PAX8 Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded mouse kidney reacted with PAX8 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Application
Western blot analysis of PAX8 Antibody in HL-60 cell line lysates (35ug/lane)
Application
Western Blot at 1:1000 dilution Lane 1: SK-OV-3 whole cell lysate Lane 2: 293 whole cell lysate Lysates/proteins at 20 ug per lane.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50 For FACS starting dilution is: 1:10~50 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
