Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Phospho-Tyr118).Application
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum using Paxillin (Phospho-Tyr118).| Product Name | Paxillin (phospho Tyr118) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | IKBA, MAD3, NFKBI, PAXI, PXN |
| Product description | Cytoskeletal protein involved in actin-membrane attachment at sites of cell adhesion to the extracellular matrix (focal adhesion).1) Tao WA, et al. (2005) Nat Methods. 2 (8): 591-598. Zhang Y, et al. (2005) Mol Cell Proteomics. 4 (9): 1240-1250. Sanders MA, et al. (2005) J Biol Chem. 280 (25): 23516-23522. Rush J, et al. (2004) Nat Biotechnol. 23 (1): 94-101. |
| Immunogen | Paxillin (Phospho-Tyr118) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 118 (H-V-Y (p) -S-F) derived from Human Paxillin. |
| Modification | p-Tyr118 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | IHCWB |
| Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PXN |
|---|---|
| Alternative Names | IKBA MAD3 NFKBI PAXI PXN |
| Molecular Weight(MW) | 68 kDa |
| Tissue Specificity | This antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 118. |
| Entrez Gene | 5829 |
|---|---|
| Protein Accession | NP_001074324.1 |
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Paxillin (Phospho-Tyr118).
Application
Western blot analysis of lysed extracts from 3T3 cells untreated or treated with serum using Paxillin (Phospho-Tyr118).| Application Notes | Western Blot: 1:500~1:1000, Immunohistochemistry: 1:50~1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
