Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Paxillin (Phospho-Tyr31).| Product Name | Paxillin (phospho Tyr31) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | NACP, PARK1, PAXI, PXN |
| Product description | An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions1) Davidson D, et al. (2001) EMBO J 20 (13): 3414-3426. Fleming I, et al. (1999) Proc Natl Acad Sci U S A 96 (3): 1123-1128. Goldberg MB. (2001) Microbiol Mol Biol Rev 65 (4): 595-626. Kook S, et al. (2000) Mol Biol Cell 11 (3): 929-939. |
| Immunogen | Paxillin (Phospho-Tyr31) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 31 (T-P-Y (p) -S-Y) derived from Human Paxillin. |
| Modification | p-Tyr31 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PXN |
|---|---|
| Alternative Names | NACP PARK1 PAXI PXN |
| Molecular Weight(MW) | 68 kDa |
| Tissue Specificity | This antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31. |
| Entrez Gene | 5829 |
|---|---|
| Protein Accession | NP_001074324.1 |
Application
Western blot analysis of lysed extracts from HT29 cells untreated or treated with PMA using Paxillin (Phospho-Tyr31).| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
