Application
Western blot analysis of PBP using rabbit polyclonal PBP Antibody (A152) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PBP gene.Application
Western blot analysis of anti-PBP Antibody in mouse NIH-3T3 tissue lysates (35ug/lane)Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.Application
Western blot analysis of anti-PBP Antibody Antibody in 293 cell line lysates (35ug/lane)| Product Name | PBP Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Phosphatidylethanolamine-binding protein 1, PEBP-1, HCNPpp, Neuropolypeptide h3, Prostatic-binding protein, Raf kinase inhibitor protein, RKIP, Hippocampal cholinergic neurostimulating peptide, HCNP, PEBP1, PBP, PEBP |
| Product description | PBP binds ATP, opioids and phosphatidylethanolamine, exhibiting a lower affinity for phosphatidylinositol and phosphatidylcholine. This serine protease inhibitor inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase. PBP contains hippocampal cholinergic neurostimulating peptide (HCNP), which may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase.1) Tohdoh, N., et al., Brain Res. Mol. Brain Res. 30(2):381-384 (1995). Hori, N., et al., Gene 140(2):293-294 (1994). Seddiqi, N., et al., J. Mol. Evol. 39(6):655-660 (1994). Moore, C., et al., Brain Res. Mol. Brain Res. 37 (1-2), 74-78 (1996). |
| Immunogen | This PBP antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 137-167 amino acids from the Central region of human PBP. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PEBP1 |
|---|---|
| Alternative Names | Phosphatidylethanolamine-binding protein 1 PEBP-1 HCNPpp Neuropolypeptide h3 Prostatic-binding protein Raf kinase inhibitor protein RKIP Hippocampal cholinergic neurostimulating peptide HCNP PEBP1 PBP PEBP |
| Molecular Weight(MW) | 21 kDa |
Application
Western blot analysis of PBP using rabbit polyclonal PBP Antibody (A152) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PBP gene.
Application
Western blot analysis of anti-PBP Antibody in mouse NIH-3T3 tissue lysates (35ug/lane)
Application
Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. BC = breast carcinoma; HC = hepatocarcinoma.
Application
Western blot analysis of anti-PBP Antibody Antibody in 293 cell line lysates (35ug/lane)| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:50~100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
