Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).| Product Name | PCBP1 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCBP1, HNRPX, HNRPE1, hnRNP-X, HEL-S-85, hnRNP-E1 |
| Product description | PCBP1 appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly (rC)-binding protein. It contains three K-homologous (KH) domains which may be involved in RNA binding. This protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. PCBP1 is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.This intronless gene is thought to be generated by retrotransposition of a fully processed PCBP-2 mRNA. This gene and PCBP-2 has paralogues PCBP3 and PCBP4 which is thought to arose as a result of duplication events of entire genes. The protein encoded by this gene appears to be multifunctional. It along with PCBP-2 and hnRNPK corresponds to the major cellular poly (rC)-binding proteins. It contains three K-homologous (KH) domains which may be involved in RNA binding. This encoded protein together with PCBP-2 also functions as translational coactivators of poliovirus RNA via a sequence-specific interaction with stem-loop IV of the IRES and promote poliovirus RNA replication by binding to its 5'-terminal cloverleaf structure. It has also been implicated in translational control of the 15-lipoxygenase mRNA, human Papillomavirus type 16 L2 mRNA, and hepatitis A virus RNA. The encoded protein is also suggested to play a part in formation of a sequence-specific alpha-globin mRNP complex which is associated with alpha-globin mRNA stability.1) Zhong, N., (2005) Biochem. Biophys. Res. Commun. 338 (2), 855-861. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCBP1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PCBP1 antibody can be used for detection of PCBP1 by ELISA at 1:62500. PCBP1 antibody can be used for detection of PCBP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PCBP1 |
|---|---|
| Alternative Names | PCBP1 HNRPX HNRPE1 hnRNP-X HEL-S-85 hnRNP-E1 |
| Molecular Weight(MW) | 39 kDa |
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in WB on HepG2 at 2.5 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PCBP1 antibody can be used for detection of PCBP1 by ELISA at 1:62500. PCBP1 antibody can be used for detection of PCBP1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PCBP1 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.Store at -20 °C.Stable for 12 months from date of receipt |
