Application
Antibody used in WB on Human NT-2 at 2 ug/ml.Application
Antibody used in IHC on Human NT-2 at 1ug/50ul.Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.Application
Antibody used in IHC on Human brain stem at: 1:50.Application
Antibody used in IHC on Human Spleen at 4.0-8.0 ug/ml.| Product Name | PCNA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PCNA, MGC8367, |
| Product description | PCNA is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the polymerase's processibility during elongation of the leading strand.The protein encoded by this gene is found in the nucleus and is a cofactor of DNA polymerase delta. The encoded protein acts as a homotrimer and helps increase the processivity of leading strand synthesis during DNA replication. In response to DNA damage, this protein is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. Two transcript variants encoding the same protein have been found for this gene. Pseudogenes of this gene have been described on chromosome 4 and on the X chromosome.1) Senga, T., (2006) J. Biol. Chem. 281 (10), 6246-6252. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PCNA. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PCNA antibody can be used for detection of PCNA by ELISA at 1:62500. PCNA antibody can be used for detection of PCNA by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | DogHumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Symbol | PCNA |
|---|---|
| Alternative Names | PCNA MGC8367 |
| Molecular Weight(MW) | 29 kDa, 29 kDa |
Application
Antibody used in WB on Human NT-2 at 2 ug/ml.
Application
Antibody used in IHC on Human NT-2 at 1ug/50ul.
Application
Antibody used in WB on Human HepG2 at 1.25 ug/ml.
Application
Antibody used in IHC on Human brain stem at: 1:50.
Application
Antibody used in IHC on Human Spleen at 4.0-8.0 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PCNA antibody can be used for detection of PCNA by ELISA at 1:62500. PCNA antibody can be used for detection of PCNA by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PCNA antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
