Application
Immunohistochemistry staining of PCNA in breast tissue using PCNA Antibody.WB
(B) The protein expression of FoxO3 in the nucleus, cytoplasm, and overall. GAPDH and proliferating cell nuclear antigen (PCNA) were used as internal controls for the cytoplasmic and nuclear fractions, respectively.| Product Name | PCNA Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | The proliferating cell nuclear antigen (PCNA) is an auxiliary protein of DNA polymerase delta and is involved in the control of eukaryotic DNA replication by increasing the processibility of DNA polymerase during elongation of the leading strand. PCNA is expressed in the nucleus of all proliferating cells and is pivotal for DNA synthesis and cell cycle progression. In response to DNA damage, PCNA is mono-ubiquitinated and is involved in mismatch-provoked excision. PCNA is a useful marker for DNA synthesis and is highly conserved among most species. |
| Immunogen | PCNA antibody was raised against an internal region of PCNA (Human). |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAIHCWB |
| PCNA antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 10 μg/mL.: |
|
| Species Reactivity | BovineChickenDogFishHumanMonkeyMouseRatXenopus |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PCNA |
|---|---|
| Alternative Names | PCNA Cyclin |
Application
Immunohistochemistry staining of PCNA in breast tissue using PCNA Antibody.
WB
(B) The protein expression of FoxO3 in the nucleus, cytoplasm, and overall. GAPDH and proliferating cell nuclear antigen (PCNA) were used as internal controls for the cytoplasmic and nuclear fractions, respectively.| Application Notes | PCNA antibody can be used in ELISA starting at 1:000 - 1:1000, and immunohistochemistry starting at 10 μg/mL.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store PCNA antibody at 4 ˚C or -20 ˚C. As with all antibodies avoid freeze/thaw cycles. |
| Storage Buffer | 0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. |
