Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody at 1:1000.Application
PCSK9 Antibody (RB18880) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Immunohistochemical analysis of paraffin-embedded H.colon carcinoma section using PCSK9 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.Application
Western blot analysis of lysate from HepG2 cell line, using PCSK9 Antibody at 1:1000.| Product Name | PCSK9 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Proprotein convertase subtilisin/kexin type 9, 3421-, Neural apoptosis-regulated convertase 1, NARC-1, Proprotein convertase 9, PC9, Subtilisin/kexin-like protease PC9, PCSK9, NARC1 |
| Product description | PCSK9 is a proprotein convertase belonging to the proteinase K subfamily of the secretory subtilase family. This protein is synthesized as a soluble zymogen that undergoes autocatalytic intramolecular processing in the endoplasmic reticulum. The protein may function as a proprotein convertase. The protein plays a role in cholesterol homeostasis and may have a role in the differentiation of cortical neurons.1) Abifadel,M., Rabes,J.P. Hum. Mutat. 30 (7), E682-E691 (2009) |
| Immunogen | This PCSK9 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 479-508 amino acids from the C-terminal region of human PCSK9. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:100 For FACS starting dilution is: 1:10~50: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Gene Symbol | PCSK9 |
|---|---|
| Alternative Names | Proprotein convertase subtilisin/kexin type 9 3421- Neural apoptosis-regulated convertase 1 NARC-1 Proprotein convertase 9 PC9 Subtilisin/kexin-like protease PC9 PCSK9 NARC1 |
| Molecular Weight(MW) | 74 kDa |
Application
Flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Western blot analysis of lysate from Hela cell line, using PCSK9 Antibody at 1:1000.
Application
PCSK9 Antibody (RB18880) IHC analysis in formalin fixed and paraffin embedded human Colon carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Immunohistochemical analysis of paraffin-embedded H.colon carcinoma section using PCSK9 Antibody . Antibody was diluted at 1:100 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.
Application
Western blot analysis of lysate from HepG2 cell line, using PCSK9 Antibody at 1:1000.| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:100 For FACS starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
Data sheet for OM287598