Application
Antibody used in WB on HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Product Name | PDCD4 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDCD4, H731, MGC33046, MGC33047 |
| Product description | PDCD4 is a protein localized to the nucleus in proliferating cells. Expression of its gene is modulated by cytokines in natural killer and T cells. PDCD4 is thought to play a role in apoptosis but the specific role has not yet been determined.This gene encodes a protein localized to the nucleus in proliferating cells. Expression of this gene is modulated by cytokines in natural killer and T cells. The gene product is thought to play a role in apoptosis but the specific role has not yet been determined. Two transcripts encoding different isoforms have been identified.1) Yang, H.S., (2006) Mol. Cell. Biol. 26 (4), 1297-1306. |
| Immunogen | Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PDCD4. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| PDCD4 antibody can be used for detection of PDCD4 by ELISA at 1:12500. PDCD4 antibody can be used for detection of PDCD4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDCD4 |
|---|---|
| Alternative Names | PDCD4 H731 MGC33046 MGC33047 |
| Molecular Weight(MW) | 50 kDa |
Application
Antibody used in WB on HepG2 at 2.0 ug/ml (Lane A: Primary Antibody and Lane B: Primary Antibody + Blocking Peptide ).
Application
Antibody used in WB on Human Jurkat 0.2-1 ug/ml.| Positive Control | 1) Cat. No. 1211 - HepG2 Cell Lysate |
|---|---|
| Application Notes | PDCD4 antibody can be used for detection of PDCD4 by ELISA at 1:12500. PDCD4 antibody can be used for detection of PDCD4 by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For short periods of storage (days) store at 4˚C. For longer periods of storage, store PDCD4 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. |
| Storage Buffer | Antibody is lyophilized in PBS buffer with 2% sucrose. Add 50 μL of distilled water. Final antibody concentration is 1 mg/mL. |
