Application
Immunocytochemistry of PDCD5 in Jurkat cells with PDCD5 antibody at 2 μg/mL.Application
Immunofluorescence of PDCD5 in Jurkat cells with PDCD5 antibody at 10 μg/mL.Application
Western blot analysis of PDCD5 in EL4 cell lysate with PDCD5 antibody at 1μg/mL.| Product Name | PDCD5 Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | PDCD5 Antibody: DCHT, PASK, SPAK, DCHT, Ste-20-related kinase |
| Product description | PDCD5 Antibody: Programmed cell death 5 (PDCD5), a human apoptosis-related protein, is thought to play an early and universal role in apoptosis. PDCD5 is widely expressed and is upregulated in cells undergoing apoptosis, where it translocates rapidly from the cytoplasm to the nucleus. PDCD5 has a compact core structure of low flexibility with two mobile alpha-helices at N-terminal and a flexible unstructured C-terminal region. The charged residues are crucial for the ability of apoptosis-promoting and cell translocation of the protein. PDCD5 can facilitate apoptosis and enhance TAJ/TROY-induced paraptosis-like cell death. PDCD5 may play a dual role in the Tip60 pathway. It interacts with Tip60 and functions as a Tip60 co-activator to promote apoptosis. The nucleotide polymorphisms in the 5'-upstream region of PDCD5 affect promoter activity and the susceptibility of a Chinese population to develop chronic myelogenous leukemia and may represent a novel tumor suppressor gene influencing lung cancer.1) Liu H, Wang Y, Zhang Y, et al. TFAR19, a novel apoptosis-related gene cloned from human leukemia cell line TF-1, could enhance apoptosis of some tumor cells induced by growth factor withdrawal. Biochem. Biophys. Res. Commun.1999; 254:203-10. |
| Immunogen | PDCD5 antibody was raised against a 21 amino acid synthetic peptide from near the carboxy terminus of human PDCD5. The immunogen is located within the last 50 amino acids of PDCD5. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAICCIFWB |
| PDCD5 antibody can be used for detection of PDCD5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | STK39 |
|---|---|
| Alternative Names | PDCD5 Antibody: DCHT PASK SPAK DCHT Ste-20-related kinase |
| Sequence Similarities | 2) Cat. No. 17-005 - Jurkat Cell Slide |
Application
Immunocytochemistry of PDCD5 in Jurkat cells with PDCD5 antibody at 2 μg/mL.
Application
Immunofluorescence of PDCD5 in Jurkat cells with PDCD5 antibody at 10 μg/mL.
Application
Western blot analysis of PDCD5 in EL4 cell lysate with PDCD5 antibody at 1μg/mL.| Positive Control | 1) Cat. No. 1287 - EL4 Cell Lysate |
|---|---|
| Application Notes | PDCD5 antibody can be used for detection of PDCD5 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 10 μg/mL.: |
| Form | Liquid |
|---|---|
| Storage Instructions | PDCD5 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | PDCD5 Antibody is supplied in PBS containing 0.02% sodium azide. |
