Application
PDE1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.Application
Western Blot at 1:1000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).| Product Name | PDE1B Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, Cam-PDE 1B, 63 kDa Cam-PDE, PDE1B, PDE1B1, PDES1B |
| Product description | Cyclic nucleotide phosphodiesterases (PDEs) catalyze hydrolysis of the cyclic nucleotides cAMP and cGMP to the corresponding nucleoside 5-prime-monophosphates. Mammalian PDEs have been classified into several families based on their biochemical properties. Members of the PDE1 family, such as PDE1B, are calmodulin (see MIM 114180)-dependent PDEs (CaM-PDEs) that are stimulated by a calcium-calmodulin complex (Repaske et al., 1992 [PubMed 1326532]).1) Bailey, S.D., et al. Diabetes Care 33(10):2250-2253(2010) |
| Immunogen | This PDE1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 473-500 amino acids from the C-terminal region of human PDE1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | Ig |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDE1B |
|---|---|
| Alternative Names | Calcium/calmodulin-dependent 3' 5'-cyclic nucleotide phosphodiesterase 1B Cam-PDE 1B 63 kDa Cam-PDE PDE1B PDE1B1 PDES1B |
| Molecular Weight(MW) | 61 kDa |
Application
PDE1B Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Application
Western Blot at 1:1000 dilution + THP-1 whole cell lysate Lysates/proteins at 20 ug per lane.
Application
Western blot analysis in mouse brain tissue lysates (35ug/lane).| Application Notes | For WB starting dilution is: 1:1000 For IHC-P starting dilution is: 1:10~50: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Storage Buffer | Supplied in PBS with 0.09% (W/V) sodium azide. |
