Application
Western blot analysis of extracts from HepG2 cells treated with EGF using Phospho-PDGFR beta (Tyr740) Antibody. The lane on the right is treated with the antigen-specific peptide.| Product Name | PDGFR beta (phospho Tyr740) Antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | CD140b, PDGF-R-beta, PDGFR, PGFRB, kinase PDGFR-beta |
| Product description | PDGF Receptor β encodes a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.1) Mitchell D, et al. (2007) J Biol Chem 282, 15606-18Wu JH, et al. (2006) J Biol Chem 281, 37758-72 . Bae YS, et al. (2000)J Biol Chem 275, 10527-31. |
| Immunogen | PDGFR beta (phospho Tyr740) antibody was raised against a peptide sequence around phosphorylation site of tyrosine 740 (G-G-Y (p)-M-D) derived from Human PDGFR beta. |
| Modification | p-Tyr740 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western Blot: 1:500~1:1000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Symbol | PDGFRB |
|---|---|
| Alternative Names | CD140b PDGF-R-beta PDGFR PGFRB kinase PDGFR-beta |
| Molecular Weight(MW) | 170 kDa |
| Tissue Specificity | The antibody detects endogenous level of PDGFR beta only when phosphorylated at tyrosine 740. |
| Entrez Gene | 5159 |
|---|---|
| Protein Accession | NP_002600.1 |
Application
Western blot analysis of extracts from HepG2 cells treated with EGF using Phospho-PDGFR beta (Tyr740) Antibody. The lane on the right is treated with the antigen-specific peptide.| Application Notes | Western Blot: 1:500~1:1000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store antibody at -20˚C for up to one year. |
| Storage Buffer | Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
